The NC1 domain of type XIX collagen inhibits melanoma cell migration.
Fiche publication
Date publication
novembre 2010
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BRASSART-PASCO Sylvie, Dr MONBOISSE Jean-Claude, Pr RAMONT Laurent, Pr SAPI Janos
Tous les auteurs :
Toubal A, Ramont L, Terryn C, Brassart-Pasco S, Patigny D, Sapi J, Monboisse JC, Maquart FX
Lien Pubmed
Résumé
Type XIX collagen is a minor collagen that localizes to basement membrane zones. We previously demonstrated that the C-terminal NC1 domain of type XIX collagen inhibits tumor growth in vivo. In the present study, we analyzed the effects of the NC1(XIX) collagen domain on migratory behaviour of melanoma B16F10 cells. We found that NC1(XIX) do not inhibit melanoma cell proliferation. On the contrary, NC1(XIX) strongly inhibited the migratory capacities of melanoma cells in the scratch wound model and in Ibidi(R) devices: cell migration speed was 7.69 +/- 1.49 mum/h for the controls vs 6.64 +/- 0.82 mum/h for cells incubated with 30 mumol/L NC1(XIX) and 5.72 +/- 0.67 mumol/h with 60 mumol/L NC1(XIX). Similar results were obtained with UACC 903 human melanoma cells. Further work will be necessary to elucidate the molecular mechanisms of this migration inhibition. It may, however, explain, at least partially, the inhibition of tumor growth that we observed in vivo.
Référence
Eur J Dermatol. 2010 Nov-Dec;20(6):712-8