Comparison of the Physicochemical Properties of Chitin Extracted from Cicada orni Sloughs Harvested in Three Different Years and Characterization of the Resulting Chitosan

Date publication

novembre 2021

Journal

Applied Sciences

Auteurs

Membres identifiés du Cancéropôle Est :
Dr CLEYMAND Franck, Pr MANO João F.

Tous les auteurs :
Poerio A, Girardet T, Petit C, Fleutot S, Jehl JP, Arab-Tehrany E, Mano JF, Cleymand F

Résumé

Chitin and its derivative chitosan are among the most used polysaccharides for biomedical and pharmaceutical applications. Most of the commercially available chitin is obtained from seafood wastes. However, the interest in alternative renewable sources of chitin and chitosan, such as insects, is growing. When new sources are identified, their stability over time has to be evaluated to allow for their commercialization. The aim of this study is to compare the physicochemical properties of chitin extracted from Cicada orni sloughs harvested in three different years (2017, 2019 and 2020) in order to assess the stability of the source and the repeatability of the extraction process. Chitin and its derivative chitosan were characterized by simple techniques such as Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Results suggest that the physicochemical properties of the extracted chitin varied from year to year, and that these differences are not due to the extraction process, but rather to intrinsic differences within the source. We showed that these differences could already be detected by analyzing the raw material (i.e., cicada sloughs) using the above-mentioned simple methods. The chitosan obtained from deacetylation of chitin had a low degree of deacetylation (66.2±1.6%). This low degree of deacetylation can be attributed to the deacetylation process, which is probably not appropriate for this source of chitin.

Mots clés

chitin; chitosan; extraction; physicochemical characterization