NTPDase2 and the P2Y1 receptor are not required for mammalian eye formation.
Fiche publication
Date publication
mars 2015
Journal
Purinergic signalling
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GACHET Christian
Tous les auteurs :
Gampe K, Haverkamp S, Robson SC, Gachet C, Hüser L, Acker-Palmer A, Zimmermann H
Lien Pubmed
Résumé
Eye formation in vertebrates is controlled by a conserved pattern of molecular networks. Homeobox transcription factors are crucially involved in the establishment and maintenance of the retina. A previous study of Massé et al. (Nature, 449: 1058-62, 2007) using morpholino knockdown identified the ectonucleotidase NTPDase2 and the P2Y1 receptor as essential elements for eye formation in embryos of the clawed frog Xenopus laevis. In order to investigate whether a similarly essential mechanism would be active in mammalian eye development, we analyzed mice KO for Entpd2 or P2ry1 as well as double KO for Entpd2/P2ry1. These mice developed normal eyes. In order to identify potential deficits in the molecular identity or in the arrangement of the cellular elements of the retina, we performed an immunohistological analysis using a variety of retinal markers. The analysis of single and double KO mice demonstrated that NTPDase2 and P2Y1 receptors are not required for murine eye formation, as previously shown for eye development in Xenopus laevis.
Mots clés
Adenosine Triphosphatases, genetics, Animals, Eye, embryology, Mice, Mice, Knockout, Organogenesis, genetics, Receptors, Purinergic P2Y1, genetics, Retina, embryology
Référence
Purinergic Signal.. 2015 Mar;11(1):155-60