Ribosomal RNA 2'--methylations regulate translation by impacting ribosome dynamics.
Fiche publication
Date publication
mars 2022
Journal
Proceedings of the National Academy of Sciences of the United States of America
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri, Dr MARCHAND Virginie
Tous les auteurs :
Khoshnevis S, Dreggors-Walker RE, Marchand V, Motorin Y, Ghalei H
Lien Pubmed
Résumé
SignificanceThe presence of RNA chemical modifications has long been known, but their precise molecular consequences remain unknown. 2'--methylation is an abundant modification that exists in RNA in all domains of life. Ribosomal RNA (rRNA) represents a functionally important RNA that is heavily modified by 2'--methylations. Although abundant at functionally important regions of the rRNA, the contribution of 2'--methylations to ribosome activities is unknown. By establishing a method to disturb rRNA 2'--methylation patterns, we show that rRNA 2'--methylations affect the function and fidelity of the ribosome and change the balance between different ribosome conformational states. Our work links 2'--methylation to ribosome dynamics and defines a set of critical rRNA 2'--methylations required for ribosome biogenesis and others that are dispensable.
Mots clés
2′-O-methylation, rRNA modification, ribosome biogenesis, ribosome dynamics, translation regulation
Référence
Proc Natl Acad Sci U S A. 2022 Mar 22;119(12):e2117334119