Analysis of Fluorescence Decay Kinetics of Indocyanine Green Monomers and Aggregates in Brain Tumor Model In Vivo.
Fiche publication
Date publication
novembre 2021
Journal
Nanomaterials (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BEZDETNAYA-BOLOTINE Lina
Tous les auteurs :
Farrakhova D, Romanishkin I, Maklygina Y, Bezdetnaya L, Loschenov V
Lien Pubmed
Résumé
Spectroscopic approach with fluorescence time resolution allows one to determine the state of a brain tumor and its microenvironment via changes in the fluorescent dye's fluorescence lifetime. Indocyanine green (ICG) is an acknowledged infra-red fluorescent dye that self-assembles into stable aggregate forms (ICG NPs). ICG NPs aggregates have a tendency to accumulate in the tumor with a maximum accumulation at 24 h after systemic administration, enabling extended intraoperative diagnostic. Fluorescence lifetime analysis of ICG and ICG NPs demonstrates different values for ICG monomers and H-aggregates, indicating promising suitability for fluorescent diagnostics of brain tumors due to their affinity to tumor cells and stability in biological tissue.
Mots clés
H-aggregates, brain cancer, fluorescent diagnosis, fluorescent dyes, fluorescent lifetime, glioma C6, indocyanine green, near-infrared range
Référence
Nanomaterials (Basel). 2021 Nov 24;11(12):