Three dimensional in vitro culture systems in anticancer drug discovery targeted on cancer stem cells.
Fiche publication
Date publication
janvier 2021
Journal
American journal of cancer research
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MULLER Christian
Tous les auteurs :
Radajewska A, Przybyszewski O, Emhemmed F, Muller CD, Barg E, Moreira H
Lien Pubmed
Résumé
Worldwide, tumors are one of the most common causes of death. Every year 3.7 million new cases occur in Europe and more than 1.9 million patients die (WHO data). Most of the fields of research are focused on developing new therapeutic strategies that will be effective in eliminating the tumor, preventing its remission, and avoiding or reducing the side effects of therapy. In the past, generally classical 2D cell cultures or immunodeficient animal models had been used to cultivate and test drugs on human cancer cell lines. Nowadays, there are increasing interests in three-dimensional (3D) cell cultures, a method with significant differences from flat cultured cells, both considering gene expressions and cell-cell interactions. Various evidence suggests that high tumorigenic properties might be dependent on the occurrence of a small cell population, pointed out to be responsible for metastasis and recurrence. This population is called cancer stem cells (CSCs), hinted to have a lot of similarities with normal stem cells. CSCs are the main reason for chemotherapy failure as well as multi-drug resistance (MDR). CSCs can also interact through the cytokine network, with other cells like the macrophages of the inflammatory system. The big advantage of a 3D culture is the possibility to isolate and investigate the CSCs population surrounded by its environment. This article aims to sum up known 3D cell cultures, especially in the field of CSCs research due to the importance of the tumor's environment on stem cell's markers expression and their development.
Mots clés
3D cell cultures, Cancer stem cells (CSC), anticancer drug discovery, cancer organoids, hanging drop (HD), lab-on-a-chip, matrix, micro fluidics, solid-phase spheroids, ultra-low attachment plates (ULA)
Référence
Am J Cancer Res. 2021 ;11(10):4931-4946