The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms.
Fiche publication
Date publication
août 2021
Journal
Cell reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DEVYS Didier, Dr REINA-SAN-MARTIN Bernardo, Dr TORA Laszlo
Tous les auteurs :
Fischer V, Plassard D, Ye T, Reina-San-Martin B, Stierle M, Tora L, Devys D
Lien Pubmed
Résumé
SAGA (Spt-Ada-Gcn5 acetyltransferase) and ATAC (Ada-two-A-containing) are two related coactivator complexes, sharing the same histone acetyltransferase (HAT) subunit. The HAT activities of SAGA and ATAC are required for metazoan development, but the role of these complexes in RNA polymerase II transcription is less understood. To determine whether SAGA and ATAC have redundant or specific functions, we compare the effects of HAT inactivation in each complex with that of inactivation of either SAGA or ATAC core subunits in mouse embryonic stem cells (ESCs). We show that core subunits of SAGA or ATAC are required for complex assembly and mouse ESC growth and self-renewal. Surprisingly, depletion of HAT module subunits causes a global decrease in histone H3K9 acetylation, but does not result in significant phenotypic or transcriptional defects. Thus, our results indicate that SAGA and ATAC are differentially required for self-renewal of mouse ESCs by regulating transcription through different pathways in a HAT-independent manner.
Mots clés
4sU labeling, ATAC, HAT-independent function, Pol II transcription, SAGA, coactivator complexes, histone acetyltransferase, mouse embryonic stem cells, newly synthesized RNA
Référence
Cell Rep. 2021 Aug 24;36(8):109598