Manipulating polyketide stereochemistry by exchange of polyketide synthase modules.
Fiche publication
Date publication
septembre 2020
Journal
Chemical communications (Cambridge, England)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr JACOB Christophe, Pr WEISSMAN Kira
Tous les auteurs :
Massicard JM, Soligot C, Weissman KJ, Jacob C
Lien Pubmed
Résumé
A key goal of modular polyketide synthase (PKS) engineering is to alter polyketide stereochemistry. Here we report that exchanging whole PKS modules is a more productive approach than swapping individual ketoreductase (KR) domains for introducing rare 'A2' and 'B2' stereochemistry into model polyketides, and identify four modular 'biobricks' for such synthetic biology efforts.
Référence
Chem. Commun. (Camb.). 2020 Sep 23;: