A versatile strategy to synthesize N-methyl-anthranilic acid-labelled glycoprobes for fluorescence-based screening assays.
Fiche publication
Date publication
août 2020
Journal
Chemical communications (Cambridge, England)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr FOURNEL-GIGLEUX Sylvie, Dr OUZZINE Mohamed, Dr VINCOURT Jean-Baptiste, Dr GULBERTI Sandrine
Tous les auteurs :
Bertin-Jung I, Robert A, Ramalanjaona N, Gulberti S, Bui C, Vincourt JB, Ouzzine M, Jacquinet JC, Lopin-Bon C, Fournel-Gigleux S
Lien Pubmed
Résumé
Here we propose a general strategy to label carbohydrates with N-methyl-anthranilic acid at the anomeric position. Through two examples, we demonstrate that the generated glycoprobes are suitable for fluorescence-based binding/competition assays. Our approach is expected to readily generate series of glycoprobes dedicated to screening assays for the discovery of drugs targeting carbohydrate-protein interactions.
Référence
Chem. Commun. (Camb.). 2020 Aug 13;: