Development and Characterization of Layer-by-Layer Coated Liposomes with Poly(L-Lysine) and Poly(L-Glutamic Acid) to Increase their Resistance in Biological Media.
Fiche publication
Date publication
juin 2020
Journal
International journal of pharmaceutics
Auteurs
Membres identifiés du Cancéropôle Est :
Dr FRISCH Benoit, Dr HEURTAULT Béatrice, Dr SPECHT Alexandre
Tous les auteurs :
Hermal F, Frisch B, Specht A, Bourel-Bonnet L, Heurtault B
Lien Pubmed
Résumé
Multilayered coated liposomes were prepared using the layer-by-layer (LbL) technique in an effort to improve their stability in biological media. The formulation strategy was based on the alternate deposition of two biocompatible and biodegradable polyelectrolytes - poly(L-lysine) (PLL) and poly(L-glutamic acid) (PGA) - on negatively charged small unilamellar vesicles (SUVs). Some parameters of the formulation process were optimized such as the polyelectrolyte concentration and the purification procedure. This optimized procedure has allowed the development of very homogeneous formulations of liposomes coated with up to 6 layers of polymers (so-called layersomes). The coating was characterized by dynamic light scattering (DLS), zeta potential measurements and Förster resonance energy transfer (FRET) between two fluorescently labeled polyelectrolytes. Studies on the stability of the formulations at 4°C in a buffered solution have shown that most structures are stable over 1 month without impacting their encapsulation capacity. In addition, fluorophore release experiments have demonstrated a better resistance of the layersomes in the presence of a non-ionic detergent (Triton X-100) as well as in the presence of phospholipase A and human plasma. In conclusion, new multilayered liposomes have been developed to increase the stability of conventional liposomes in biological environments.
Mots clés
Layer-by-layer coating, liposomes, poly(L-glutamic acid), poly(L-lysine), polyelectrolytes, stability
Référence
Int J Pharm. 2020 Jun 24;:119568