A limiting factor mediates the differential activation of promoters by the human progesterone receptor isoforms.
Fiche publication
Date publication
mai 1992
Journal
The Journal of biological chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GRONEMEYER Hinrich, Mr LEROUGE Thierry
Tous les auteurs :
Meyer ME, Quirin-Stricker C, Lerouge T, Bocquel MT, Gronemeyer H
Lien Pubmed
Résumé
The two transcription activation functions (TAFs) of the human progesterone receptor (hPR) have been characterized. TAF-1, located in the N-terminal region A/B, has been narrowed down to a 91-amino acid sequence, which is sufficient for transcription activation in chimeras with the GAL4 DNA binding domain. Both hPR TAF-1 and TAF-2 activate a minimal promoter in yeast. No autonomous TAF could be found in the N-terminal 164 amino acids (designated AB3) which are responsible for the differential activation of promoters by the hPR isoforms A and B. Reduction of the target gene promoter complexity did not alter this differential activation, indicating that AB3 does not require additional promoter-bound factors to exert its effect. Instead, the cell specificity of AB3 and its ability to squelch hPR-induced transcription suggest that this differential isoform activity is due to the effect of a limiting factor which binds to region AB3.
Mots clés
Amino Acid Sequence, Base Sequence, DNA, genetics, Genes, Fungal, Humans, Molecular Sequence Data, Promoter Regions, Genetic, Receptors, Progesterone, metabolism, Recombinant Proteins, genetics, Saccharomyces cerevisiae, genetics, Sequence Alignment, Transcription Factors, metabolism, Transcription, Genetic, beta-Galactosidase, metabolism
Référence
J. Biol. Chem.. 1992 May;267(15):10882-7