Platelet preparation for function testing in the laboratory and clinic: Historical and practical aspects.
Fiche publication
Date publication
octobre 2019
Journal
Research and practice in thrombosis and haemostasis
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GACHET Christian
Tous les auteurs :
Hechler B, Dupuis A, Mangin PH, Gachet C
Lien Pubmed
Résumé
Laboratory tests of platelet function are instrumental in studying platelet physiology and inherited or acquired platelet abnormalities. Light transmission aggregometry, developed in the early 1960s, is still considered the gold standard for the identification and diagnosis of platelet function disorders. Since then, novel techniques have been developed, including flow-based assays and flow cytometry. In this tutorial, we describe the basic methodologies for the preparation of citrated platelet-rich plasma and washed platelet suspensions and discuss their respective advantages and limitations as well as important factors to consider to perform high-quality tests of platelet function. In addition, the methodologies of the main platelet function tests (light transmission aggregation, flow-based assays, and flow cytometric assays) are described, and their respective strengths and limitations are discussed to assess various aspects of platelet biology.
Mots clés
flow cytometry, hemostasis, platelet aggregation, platelet function tests, platelet‐rich plasma
Référence
Res Pract Thromb Haemost. 2019 Oct;3(4):615-625