Selective analysis of antitumor drug interaction with living cancer cells as probed by surface-enhanced Raman spectroscopy.
Fiche publication
Date publication
janvier 1991
Journal
European biophysics journal : EBJ
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MORJANI Hamid, Pr NABIEV Igor
Tous les auteurs :
Nabiev IR, Morjani H, Manfait M
Lien Pubmed
Résumé
A new technique for the selective measurement of small amounts of antitumor drugs in the nucleus and cytoplasm of a living cancer cell, based on surface-enhanced Raman spectroscopy (SERS), is proposed. The ability to detect SERS signals from very dilute (up to 10(-10) M) solutions of doxorubicin or adriamycin (DOX), and 4'O-tetrahydropyranyl-adriamycin (THP-ADM), as well as from their complexes with targets in vitro and in vivo, has been demonstrated. SERS spectra were obtained from a population as well as from single living erythroleukaemic K562 cells treated with DOX. The results of the measurements on the population of cells containing DOX in nuclei or in the cytoplasm are well correlated with the microscopic SERS measurements on the single cells treated with DOX, obtained by selectively recording signals from the living cell nucleus or from the cytoplasm. Possibilities for the application of this new technique in different aspects of cancer research are discussed.
Mots clés
Antineoplastic Agents, analysis, Cell Nucleus, drug effects, Citrates, Citric Acid, Doxorubicin, analogs & derivatives, Humans, Silver Nitrate, Spectrum Analysis, Raman, Tumor Cells, Cultured, chemistry
Référence
Eur. Biophys. J.. 1991 ;19(6):311-6