Doxorubicin Loaded Thermo-responsive Superparamagnetic Nanocarriers for Controlled Drug Delivery and Magnetic Hyperthermia Applications.
Fiche publication
Date publication
juillet 2019
Journal
ACS applied materials & interfaces
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BEGIN-COLIN Sylvie, Dr BEZDETNAYA-BOLOTINE Lina, Pr SCHNEIDER Raphaël, Dr MERTZ Damien, Pr ALEM-MARCHAND Halima
Tous les auteurs :
Ferjaoui Z, Jamal Al Dine E, Jandayeva A, Bezdetnaya L, Soon Chang C, Schneider R, Mutelet F, Mertz D, Begin-Colin S, Quilès F, Gaffet E, Alem H
Lien Pubmed
Résumé
The present study reports on the development of thermo-responsive core/shell magnetic nanoparticles (MNPs) based on an iron oxide core and a thermo-responsive copolymer shell composed of 2-(2-methoxy) ethyl methacrylate (MEO2MA) and oligo (ethyleneglycol) methacrylate (OEGMA) moieties. Those smart nano-objects combine the magnetic properties of the core and the drug carrier properties of the polymeric shell. The loading of the anticancer drug doxorubicin (DOX) in the thermo-responsive MNPs via supramolecular interactions provides advanced features for the targeted delivery of DOX, which offers spatial and temporal control over the release of DOX. The iron oxide cores exhibit a superparamagnetic behavior with a saturation magnetization around 30 emu/g. The drug release experiments confirmed that only a small amount of DOX was released at room temperature, while almost 100% drug release was achieved after 52 h at 42°C for the Fe3O4@P(MEO2MA60OEGMA40) which exhibits an LCST of 41°C in culture media. Our data fit very well with the first order kinetic model. Moreover, the MNPs exhibit hyperthermia properties. Finally, the cytotoxicity towards human cancer SKOV-3 cells of the core/shell NPs was tested. The results showed that the polymer-capped NPs exhibited almost no toxicity at concentrations up to 12 µg.mL-1 while when loaded with DOX, a higher cytotoxicity and a decreased SKOV-3 cancer cell viability in a short time were observed. From the results obtained, it can be concluded that this smart superparamagnetic nanocarriers are promising for applications in multi-modal cancer therapy, with stealth properties and capable of delivering drug to the tumor.
Référence
ACS Appl Mater Interfaces. 2019 Jul 30;: