[Culture conditions for gametes and embryos: Which culture medium? Which impact on newborn?]
Fiche publication
Date publication
mai 2018
Journal
Gynecologie, obstetrique, fertilite & senologie
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GUEANT Jean-Louis, Dr KOSCINSKI Isabelle
Tous les auteurs :
Koscinski I, Merten M, Kazdar N, Guéant JL
Lien Pubmed
Résumé
Many studies have examined the impact of cell/embryo culture media on the development of human embryo during IVF process, but few studies have followed up and compared the effects of these culture media on the developmental outcome of children conceived by IVF. As recurrent experimental evidence from animal studies suggests potential long-term effects of embryo culture media on the health outcome of IVF-conceived children, more studies are needed to clarify the role of the culture media and mechanisms underlying such effects. In human, however, the effects of culture media are difficult to pinpoint due to complications stem from both the influence of maternal nutrition during the gestational period and the parental genetic. Based on a simple review of the literature integrating animal experimentations and human clinic studies, we suggest that the composition of culture medium should be considered beyond the character of unique or sequential medium, corresponding to "let embryo choose" or "back to nature" respectively. Instead, we suggest that the main components of embryo culture media should be considered from the point of view of metabolic consequences and potential epigenetic effects. Given that energetic metabolites can regulate epigenetic machinery, we hypothesize that metabolic abnormalities linked to morphological abnormalities could reveal epigenetic defects in embryos.
Mots clés
Culture medium, Embryo epigenetics, Embryo metabolism, IVF children follow-up, Milieu de culture, Métabolisme embryonnaire, Suivi des enfants FIV, Épigénétique embryonnaire
Référence
Gynecol Obstet Fertil Senol. 2018 May;46(5):474-480