Design and evaluation of histidine-rich amphipathic peptides for siRNA delivery.
Fiche publication
Date publication
juillet 2010
Journal
Pharmaceutical research
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BECHINGER Burkhard, Dr KICHLER Antoine
Tous les auteurs :
Langlet-Bertin B, Leborgne C, Scherman D, Bechinger B, Mason AJ, Kichler A
Lien Pubmed
Résumé
Short linear peptides have a high potential for delivering various drugs with therapeutic potential, including nucleic acids. Recently, we have shown that the cationic amphipathic histidine-rich peptide LAH4 (KKALLALALHHLAHLALHLALALKKA) possesses high plasmid DNA delivery capacities. Since such peptides are thought to efficiently disrupt endosomal membranes, we have tested their ability to deliver small interfering RNA (siRNA) into mammalian cells.
Mots clés
Amino Acid Sequence, Cell Line, Drug Delivery Systems, Drug Design, Histidine, chemistry, Humans, Molecular Sequence Data, Peptides, administration & dosage, RNA, Small Interfering, administration & dosage
Référence
Pharm. Res.. 2010 Jul;27(7):1426-36