Polydiacetylenic nanofibers as new siRNA vehicles for in vitro and in vivo delivery.
Fiche publication
Date publication
janvier 2018
Journal
Nanoscale
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LINDNER Véronique, Dr MASSFELDER Thierry, Dr REMY Jean-Serge, Dr WAGNER Alain, Dr KICHLER Antoine
Tous les auteurs :
Neuberg P, Hamaidi I, Danilin S, Ripoll M, Lindner V, Nothisen M, Wagner A, Kichler A, Massfelder T, Remy JS
Lien Pubmed
Résumé
Polydiacetylenic nanofibers (PDA-Nfs) obtained by photopolymerization of surfactant 1 were optimized for intracellular delivery of small interfering RNAs (siRNAs). PDA-Nfs/siRNA complexes efficiently silenced the oncogene Lim-1 in the renal cancer cells 786-O in vitro. Intraperitoneal injection of PDA-Nfs/siLim1 downregulated Lim-1 in subcutaneous tumor xenografts obtained with 786-O cells in nude mice. Thus, PDA-Nfs represent an innovative system for in vivo delivery of siRNAs.
Mots clés
Animals, Cell Line, Tumor, Gene Silencing, Injections, Intraperitoneal, Kidney Neoplasms, therapy, LIM-Homeodomain Proteins, metabolism, Mice, Mice, Nude, Nanofibers, Polyacetylene Polymer, RNA, Small Interfering, administration & dosage, Transcription Factors, metabolism, Xenograft Model Antitumor Assays
Référence
Nanoscale. 2018 Jan 25;10(4):1587-1590