Photodynamic properties of meta-tetra(hydroxyphenyl)chlorin in human tumor cells.
Fiche publication
Date publication
octobre 1999
Journal
Radiation research
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BEZDETNAYA-BOLOTINE Lina
Tous les auteurs :
Melnikova VO, Bezdetnaya LN, Potapenko AY, Guillemin F
Lien Pubmed
Résumé
The photodynamic properties of a second-generation photodynamic sensitizer, meta-tetra(hydroxyphenyl)chlorin (mTHPC) were studied by dye-sensitized photoinactivation (650 nm) of HT29 human adenocarcinoma cells in culture. The photocytotoxicity of mTHPC in vitro depended on the presence of molecular oxygen. A strong inhibition of the photocytotoxicity of mTHPC was observed upon addition of sodium azide, a known singlet oxygen quencher. Photocytotoxicity was not inhibited by scavengers of superoxide anion radical, hydrogen peroxide and hydroxyl radicals. We suggest that mTHPC photosensitizes cell killing predominantly by type II, singlet oxygen-mediated photodynamic reactions. Illumination of cells preloaded with mTHPC induced peroxidation of membrane lipids. Inhibition of photoperoxidation by alpha-tocopherol (0.1 mM) present during illumination did not result in any decrease in toxicity, suggesting that reactions of lipid peroxidation play only a minor role in the overall photocytotoxic effect of mTHPC.
Mots clés
Cell Survival, drug effects, Ferricyanides, pharmacology, Free Radical Scavengers, pharmacology, HT29 Cells, Humans, Lipid Peroxidation, Mesoporphyrins, pharmacokinetics, Photochemotherapy, Photosensitizing Agents, pharmacokinetics, Reactive Oxygen Species
Référence
Radiat. Res.. 1999 Oct;152(4):428-35