Detection of disseminated tumor cells in aspirative drains after neck dissection.
Fiche publication
Date publication
janvier 2015
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BEZDETNAYA-BOLOTINE Lina, Pr DOLIVET Gilles, Pr FAURE Gilbert, Pr MERLIN Jean-Louis, Dr CORTESE Sophie, Dr GUILLET-THIBAULT Julie, Dr MASTRONICOLA Romina
Tous les auteurs :
Mastronicola R, Berteau C, Tu Q, Cortese S, Guillet J, Phulpin B, Gangloff P, Bezdetnaya L, Merlin JL, Faure G, Dolivet G
Lien Pubmed
Résumé
The dissemination of individual tumor cells is a common phenomenon in solid cancers. Detection of tumor cells in bone marrow disseminated tumor cells (DTC) and in peripheral blood circulating tumor cells (CTC) in nonmetastatic situation is of high prognostic significance. Compared to breast, colon and prostate cancers, the studies on CTC and DTC in head and neck cancers are sparse. The objective of our study was to detect DTC in drains after neck dissection. Fourteen patients undergoing surgery for stages III and IV head and neck cancers were enrolled in this study-twelve presenting with squamous cell carcinoma and two with adenocarcinoma. Redon drain analysis was performed by the Cellsearch method using immunomagnetic and fluorescence approaches. A positivity threshold value was set at 2DTC/7.5 ml of the sample. Tumor cells were detected in drains of 69 % of patients a few days after surgery. The range of quantification was 3-2,094 DTC/5 ml and we showed morphological differences between the two types of carcinoma cells. DTC were detected after neck dissection both in squamous cell carcinoma and in adenocarcinoma. Potential clinical significance of tumor cells needs to be further investigated as their presence could affect pre-surgical and post-operative treatments.
Référence
Eur Arch Otorhinolaryngol. 2015 Jan 30.