An alternative scheme for the multiplexed acquisition of 1D and 2D NMR spectra.
Fiche publication
Date publication
septembre 2010
Auteurs
Membres identifiés du Cancéropôle Est :
Dr NUZILLARD Jean-Marc
Tous les auteurs :
Plainchont B, Martinez A, Tisse S, Bouillon JP, Wieruszeski JM, Lippens G, Jeannerat D, Nuzillard JM
Lien Pubmed
Résumé
Spin system selective 1D (1)H, 2D DQF-COSY and 2D HSQC NMR spectra were recorded in order to fully assign the (1)H and (13)C 1D NMR spectra of an asymmetrical beta-cyclodextrin derivative. Instead of individually accessing the seven sugar anomeric protons by means of long multiplet selective pulses, only short region selective pulses were used. The simultaneously selected anomeric protons were differentiated by allowing their magnetization to evolve under the sole effect of the chemical shift interaction. In each experiment, the seven recorded spectra were linear combinations of the seven desired ones. The combination coefficients were measured and used to obtain almost perfectly separated sugar unit sub-spectra. This multiplexed acquisition scheme resulted in a time gain factor of about 2.
Référence
J Magn Reson. 2010 Sep;206(1):68-73