Position-referenced microscopy for live cell culture monitoring.

Fiche publication


Date publication

janvier 2011

Auteurs

Membres identifiés du Cancéropôle Est :
Pr PRETET Jean-Luc, Dr SANDOZ Patrick


Tous les auteurs :
Galeano Z JA, Sandoz P, Gaiffe E, Launay S, Robert L, Jacquot M, Hirchaud F, Pretet JL, Mougin C

Résumé

Position-referenced microscopy (PRM) is based on smart sample holders that integrate a position reference pattern (PRP) in their depth, allowing the determination of the lateral coordinates with respect to the sample-holder itself. Regions of interest can thus be retrieved easily after culture dish transfers from a cell incubator to the microscope stage. Images recorded at different instants in time are superimposed in a common coordinate system with subpixel accuracy. This paper presents such smart Petri culture dishes and their use for live cell culture monitoring. The impact of the PRP on the light budget is discussed and performances are demonstrated. First results on the application of PRM to the observation of apoptotic body internalization are reported.

Référence

Biomed Opt Express. 2011 Apr 25;2(5):1307-18.