A Method to Determine the Spatial Scale Implicated in Adhesion. Application on Human Cell Adhesion on Fractal Isotropic Rough Surfaces.
Fiche publication
Date publication
janvier 2011
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ANSELME Karine
Tous les auteurs :
Bigerelle M, Mazeran PE, Gong W, Giljean S, Anselme K
Lien Pubmed
Résumé
An extensive statistical analysis is proposed to determine the best relevant roughness parameters as a function of spatial scales that affect adhesion on surfaces. The methodology is based on a multiscale decomposition of the roughness surface and is linked with adhesion measurements. This method is applied to study cell adhesion on a very wide range of roughnesses of titanium substrates (22 surfaces, the average roughness R-a from 1 to 21 mu m) tooled by an electro-erosion process and coated with a polyelectrolyte, that leads to identical surface chemistry. It is shown that the scale length of observation should be a few times the cell size to put into evidence the influence of the surface morphology on cell adhesion. It is observed that the adhesion is the lowest when the distance between the asperities of the roughness is near the cell size.
Référence
. 2011;87(7-8):644-70.