Position-referenced microscopy for live cell culture monitoring.
Fiche publication
Date publication
mai 2011
Auteurs
Membres identifiés du Cancéropôle Est :
Pr PRETET Jean-Luc, Dr SANDOZ Patrick
Tous les auteurs :
Galeano JAZ, Sandoz P, Gaiffe E, Launay S, Robert L, Jacquot M, Hirchaud F, Pretet JL, Mougin C
Lien Pubmed
Résumé
Position-referenced microscopy (PRM) is based on smart sample holders that integrate a position reference pattern (PRP) in their depth, allowing the determination of the lateral coordinates with respect to the sample-holder itself. Regions of interest can thus be retrieved easily after culture dish transfers from a cell incubator to the microscope stage. Images recorded at different instants in time are superimposed in a common coordinate system with subpixel accuracy. This paper presents such smart Petri culture dishes and their use for live cell culture monitoring. The impact of the PRP on the light budget is discussed and performances are demonstrated. First results on the application of PRM to the observation of apoptotic body internalization are reported. (C) 2011 Optical Society of America
Référence
. 2011 May 1;2(5):1307-18.