Detection of variable levels of RARalpha and RARgamma proteins in pluripotent and differentiating mouse embryonal carcinoma and mouse embryonic stem cells.
Fiche publication
Date publication
octobre 2011
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ROCHETTE-EGLY Cécile
Tous les auteurs :
Buchanan FQ, Rochette-Egly C, Asson-Batres MA
Lien Pubmed
Résumé
Pluripotent mouse embryonal carcinoma (mEC) and mouse embryonic stem (mES) cells differentiate into several cell lineages upon retinoic acid (RA) addition. Differentiation is facilitated, in part, by RA activation of nuclear RA receptors (RARs) that bind to DNA response elements located in the promoters of target genes. The purpose of the studies reported here was to immunolocalize RARalpha and RARgamma protein in mEC and mES cells and in their RA-induced differentiated progeny. Fixed cells were reacted with three different RARalpha antibodies and one RARgamma antibody. Pluripotent and differentiated mEC and mES cells showed positive nuclear immunoreactivity with all antibodies tested. Two RARalpha antibodies also showed positive reactivity in the cytoplasm. Surprisingly, our results revealed variability in immunofluorescence intensity and in RARalpha and RARgamma distribution from one cell to the other, suggesting that RARalpha and RARgamma protein levels were not synchronous throughout the cell population. The results indicate that RARalpha and RARgamma are present in pluripotent and differentiating mEC and mES cells and suggest that the expression of these proteins is dynamic.
Référence
Cell Tissue Res. 2011 Oct;346(1):43-51