Centromeric chromatin and its dynamics in plants.
Fiche publication
Date publication
mai 2015
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SCHMIT Anne-Catherine
Tous les auteurs :
Lermontova I, Sandmann M, Mascher M, Schmit AC, Chaboute ME
Lien Pubmed
Résumé
Centromeres are chromatin structures required for the proper segregation of chromosomes during mitosis and meiosis. The centromere is composed of centromeric DNA, often enriched in satellite repeats, and kinetochore complex proteins. To date, over 100 kinetochore components have been identified in different eukaryotes. Kinetochore assembly begins with the incorporation of centromeric histone H3 variant CENH3 into centromeric nucleosomes. Protein components of the kinetochore are either present at centromeres throughout the cell cycle or localize to centromeres transiently, only prior to the attachment of microtubules to each kinetochore in prometaphase of mitotic cells. This is the case for the spindle assembly checkpoint (SAC) proteins in animal cells. The SAC complex ensures an equal separation of chromosomes between daughter nuclei by preventing the anaphase onset before metaphase is complete, i.e., the sister kinetochores of all chromosomes are attached to spindle fibers from opposite poles. In this review we focus on the organization of centromeric DNA and the kinetochore assembly in plants. We summarize recent advances on the loading of CENH3 into the centromere, subcellular localization and protein-protein interactions of A. thaliana proteins involved in the kinetochore assembly and function. We describe the transcriptional activity of corresponding genes based on in silico analysis of their promoters and cell-cycle-dependent expression. Additionally, barley homologues of all selected A. thaliana proteins have been identified in silico and their sequences and domain structures are presented. This article is protected by copyright. All rights reserved.
Référence
Plant J. 2015 May 14