Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins.
Fiche publication
Date publication
décembre 2011
Auteurs
Membres identifiés du Cancéropôle Est :
Dr RUFF Marc
Tous les auteurs :
Jester BC, Drillien R, Ruff M, Florentz C
Lien Pubmed
Résumé
Production of recombinant protein in mammalian cells is time-consuming, labor-intensive and costly. While seeking to overcome these limitations, we discovered that Vaccinia virus has the innate ability to transfer exogenous plasmid DNA into mammalian cells during the infection process. Parameters influencing the efficiency of this event were characterized and a quick, simple and inexpensive way to produce eukaryotic proteins was established.
Référence
J Biotechnol. 2011 Dec 10;156(3):211-3