A FRET-based probe with a chemically deactivatable quencher.
Fiche publication
Date publication
mars 2012
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves, Dr WAGNER Alain
Tous les auteurs :
Leriche G, Budin G, Darwich Z, Weltin D, Mely Y, Klymchenko AS, Wagner A
Lien Pubmed
Résumé
A new concept of a chemically deactivatable quencher is proposed for a FRET-based probe that turns-on its fluorescence by either an enzymatic cleavage or a chemical reagent (sodium dithionite). This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted probes in cell experiments.
Référence
Chem Commun (Camb). 2012 Mar 28;48(26):3224-6