Microfluidity mapping using fluorescence correlation spectroscopy: a new way to investigate plasma membrane microorganization of living cells.
Fiche publication
Date publication
novembre 2012
Auteurs
Lien Pubmed
Résumé
Diffusion time distribution analysis has been employed to highlight the microfluidity fingerprint of plasma membrane of living cells. Diffusion time measurements were obtained through fluorescence correlation spectroscopy performed at the single cell level, over various eukaryotic cell lines (MCF7, LR73, KB3.1, MESSA and MDCKII). The nonsymmetric profile of the diffusion time distributions established experimentally, is discussed according to Monte Carlo simulations, which reproduce the diffusion of the fluorescent probe in heterogeneous membrane.
Référence
Biochim Biophys Acta. 2012 Nov;1818(11):2477-85