Value of HIV patients with regular follow-up as in-house internal controls of flow cytometry measurement of lymphocyte subsets.
Fiche publication
Date publication
juillet 2013
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DE CARVALHO BITTENCOURT Marcelo, Pr FAURE Gilbert
Tous les auteurs :
de Carvalho Bittencourt M, Kohler C, Henard S, Rabaud C, Bene MC, Faure GC
Lien Pubmed
Résumé
Background. Quality assessment in flow cytometry cannot obey the same rules as those applicable to the measurement of chemical analytes. However, regular follow-up of known patients may provide a robust in-house control of cell subsets evaluation. Methods. Sequential blood samples assessed for 32 HIV patients over several years and showing good stability were retrospectively assessed to establish coefficient of variations of the percentages of CD3+, CD4+, CD8+ cells and CD4+ absolute counts. Results. Mean relative standard variations for the whole cohort were of 0.04, 0.14, 0.08 and 0.18 for CD3%, CD4% CD8% and CD4 absolute counts respectively. Discussion. In-house follow up of regularly checked compliant patients is a good alternative to traditional and costly repeatability and reproducibility studies for the validation of routine flow cytometry. (c) 2013 Clinical Cytometry Society.
Référence
Cytometry B Clin Cytom. 2013 Jul 8