Expression and Purification of RNA-Protein Complexes in Escherichia coli.
Fiche publication
Date publication
janvier 2015
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DARDEL Frédéric
Tous les auteurs :
El Khouri M, Catala M, Seijo B, Chabal J, Tisné C, Dardel F, Ponchon L
Lien Pubmed
Résumé
For structural, biochemical or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended the "tRNA scaffold" method to RNA/protein co-expression in order to express and purify RNA by affinity in native condition. As a proof-of-concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein.
Mots clés
Escherichia coli, genetics, Gene Expression, Nucleic Acid Conformation, Protein Binding, Proteins, genetics, RNA, chemistry, RNA, Transfer, chemistry
Référence
Methods Mol Biol. 2015 ;1316:25-31