The histone deacetylase 9 gene encodes multiple protein isoforms.

Fiche publication


Date publication

mai 2003

Journal

The Journal of biological chemistry

Auteurs

Membres identifiés du Cancéropôle Est :
Dr GUIDEZ Fabien


Tous les auteurs :
Petrie K, Guidez F, Howell L, Healy L, Waxman S, Greaves M, Zelent A

Résumé

Histone deacetylases (HDACs) perform an important function in transcriptional regulation by modifying the core histones of the nucleosome. We have now fully characterized a new member of the Class II HDAC family, HDAC9. The enzyme contains a conserved deacetylase domain, represses reporter activity when recruited to a promoter, and utilizes histones H3 and H4 as substrates in vitro and in vivo. HDAC9 is expressed in a tissue-specific pattern that partially overlaps that of HDAC4. Within the human hematopoietic system, expression of HDAC9 is biased toward cells of monocytic and lymphoid lineages. The HDAC9 gene encodes multiple protein isoforms, some of which display distinct cellular localization patterns. For example, full-length HDAC9 is localized in the nucleus, but the isoform lacking the region encoded by exon 7 is in the cytoplasm. HDAC9 interacts and co-localizes in vivo with a number of transcriptional repressors and co-repressors, including TEL and N-CoR, whose functions have been implicated in the pathogenesis of hematological malignancies. These results suggest that HDAC9 plays a role in hematopoiesis; its deregulated expression may be associated with some human cancers.

Mots clés

Alternative Splicing, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Exons, Gene Expression Regulation, Enzymologic, Histone Deacetylases, genetics, Humans, Isoenzymes, genetics, Molecular Sequence Data, Neoplasms, enzymology, Organ Specificity, RNA, Messenger, analysis, Repressor Proteins, genetics, SUMO-1 Protein, metabolism, Ubiquitins, metabolism

Référence

J Biol Chem. 2003 05 2;278(18):16059-72