Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry.
Fiche publication
Date publication
octobre 2023
Journal
STAR protocols
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GHIRINGHELLI François, Dr LIMAGNE Emeric, Dr THIBAUDIN Marion
Tous les auteurs :
Daumoine S, Hampe L, Limagne E, Ghiringhelli F, Thibaudin M
Lien Pubmed
Résumé
Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and activation and labeling of the total cells (TILs and whole blood cells). For complete details on the use and execution of this protocol, please refer to Thibaudin et al..
Mots clés
Cancer, Flow Cytometry, Health Sciences, Immunology
Référence
STAR Protoc. 2023 10 31;4(4):102658