Advances in methods for tRNA sequencing and quantification.
Fiche publication
Date publication
décembre 2023
Journal
Trends in genetics : TIG
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri
Tous les auteurs :
Padhiar NH, Katneni U, Komar AA, Motorin Y, Kimchi-Sarfaty C
Lien Pubmed
Résumé
In the past decade tRNA sequencing (tRNA-seq) has attracted considerable attention as an important tool for the development of novel approaches to quantify highly modified tRNA species and to propel tRNA research aimed at understanding the cellular physiology and disease and development of tRNA-based therapeutics. Many methods are available to quantify tRNA abundance while accounting for modifications and tRNA charging/acylation. Advances in both library preparation methods and bioinformatic workflows have enabled developments in next-generation sequencing (NGS) workflows. Other approaches forgo NGS applications in favor of hybridization-based approaches. In this review we provide a brief comparative overview of various tRNA quantification approaches, focusing on the advantages and disadvantages of these methods, which together facilitate reliable tRNA quantification.
Mots clés
bioinformatics, hybridization, modifications, quantification, sequencing, tRNA
Référence
Trends Genet. 2023 12 19;: