Value of HIV patients with regular follow-up as in-house internal controls of flow cytometry measurement of lymphocyte subsets.
Fiche publication
Date publication
septembre 2013
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DE CARVALHO BITTENCOURT Marcelo, Pr FAURE Gilbert
Tous les auteurs :
de Carvalho Bittencourt M, Kohler C, Henard S, Rabaud C, Bene MC, Faure GC
Lien Pubmed
Résumé
BACKGROUND: Quality assessment in flow cytometry cannot obey the same rules as those applicable to the measurement of chemical analytes. However, regular follow-up of known patients may provide a robust in-house control of cell subsets evaluation. METHODS: Sequential blood samples assessed for 32 HIV patients over several years and showing good stability were retrospectively assessed to establish coefficient of variations of the percentages of CD3+, CD4+, CD8+ cells, and CD4+ absolute counts (ACs). RESULTS: Mean relative standard variations for the whole cohort were of 0.04, 0.14, 0.08, and 0.18 for CD3%, CD4%, CD8%, and CD4 ACs, respectively. DISCUSSION: In-house follow-up of regularly checked compliant patients is a good alternative to traditional and costly repeatability and reproducibility studies for the validation of routine flow cytometry. (c) 2013 International Clinical Cytometry Society.
Référence
Cytometry B Clin Cytom. 2013 Sep-Oct;84(5):324-6