[Flow cytometry in chronic lymphproliferative disorders: report of a survey on practices on behalf of CytHem].
Fiche publication
Date publication
juillet 2023
Journal
Annales de biologie clinique
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LESESVE Jean-François
Tous les auteurs :
Mayeur-Rousse C, Bouyer S, Lesesve JF, Le Garff-Tavernier M, Baseggio L
Lien Pubmed
Résumé
Flow cytometric immunophenotyping is nowadays an essential tool for diagnosis, classification and monitoring of chronic lymphoproliferative disorders (CLPD). Several recommendations on multicolor panels have been proposed in the literature but little is known about their application in routine laboratories. The CytHem group (Cytométrie Hématologique francophone), created in 2018, is organized in multiple thematic groups: among them one is dedicated to CLPD, "Cythem-SLP". The first objective of Cythem-SLP was to conduct an investigation on current practices about flow cytometry and CLPD among its members. The answers of 40 centers have been collected and investigated. Only a few of them directly apply panels proposals from the literature. Nevertheless, this investigation highlights some antibodies which are necessary for the CLPD diagnosis according to the experience of the centres. Finally, members of CytHem-SLP group are still on demand for harmonization panels proposals and interlaboratory controls.
Mots clés
flow cytometry, hematology, lymphoproliferative disorders, survey on pratices
Référence
Ann Biol Clin (Paris). 2023 07 21;81(3):289-303