Transcription factor IID parks and drives preinitiation complexes at sharp or broad promoters.
Fiche publication
Date publication
août 2023
Journal
Trends in biochemical sciences
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DEVYS Didier, Dr TORA Laszlo
Tous les auteurs :
Bernardini A, Hollinger C, Willgenss D, Müller F, Devys D, Tora L
Lien Pubmed
Résumé
Core promoters are sites where transcriptional regulatory inputs of a gene are integrated to direct the assembly of the preinitiation complex (PIC) and RNA polymerase II (Pol II) transcription output. Until now, core promoter functions have been investigated by distinct methods, including Pol II transcription initiation site mappings and structural characterization of PICs on distinct promoters. Here, we bring together these previously unconnected observations and hypothesize how, on metazoan TATA promoters, the precisely structured building up of transcription factor (TF) IID-based PICs results in sharp transcription start site (TSS) selection; or, in contrast, how the less strictly controlled positioning of the TATA-less promoter DNA relative to TFIID-core PIC components results in alternative broad TSS selections by Pol II.
Mots clés
RNA polymerase II, core promoter architecture, preinitiation complex, transcription factor IID, transcription initiation, transcription start site selection
Référence
Trends Biochem Sci. 2023 08 11;: