The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly.
Fiche publication
Date publication
août 2023
Journal
Trends in biochemical sciences
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HAMICHE Ali
Tous les auteurs :
Kale S, Boopathi R, Belotti E, Lone IN, Graies M, Schröder M, Petrova M, Papin C, Bednar J, Ugrinova I, Hamiche A, Dimitrov S
Lien Pubmed
Résumé
CENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognition. We summarize the data on the structural organization of the CENP-A nucleosome and the binding of CENP-C and CENP-N. The latter posits an apparent contradiction in engaging the CENP-A nucleosome versus the CCAN. We propose a reconciliatory model for the assembly of CCAN on centromeric chromatin.
Mots clés
CCAN complex, CENP-A nucleosome, chromatin, histone variants, mitosis
Référence
Trends Biochem Sci. 2023 08 16;: