Scale-Up of Academic Mesenchymal Stromal Cell Production.
Fiche publication
Date publication
juin 2023
Journal
Journal of clinical medicine
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BENSOUSSAN Danièle, Dr BONNET Céline, Pr DECOT Véronique, Dr REPPEL Loïc, Dr DE ISLA Natalia
Tous les auteurs :
Laroye C, Gauthier M, Morello J, Charif N, Cannard VL, Bonnet C, Lozniewski A, Tchirkov A, De Isla N, Decot V, Reppel L, Bensoussan D
Lien Pubmed
Résumé
Many clinical trials have reported the use of mesenchymal stromal cells (MSCs) following the indication of severe SARS-CoV-2 infection. However, in the COVID19 pandemic context, academic laboratories had to adapt a production process to obtain MSCs in a very short time. Production processes, especially freezing/thawing cycles, or culture medium have impacts on MSC properties. We evaluated the impact of an intermediate cryopreservation state during MSC culture to increase production yields.
Mots clés
Wharton’s jelly, mesenchymal stem cells, scale-up
Référence
J Clin Med. 2023 06 30;12(13):