Approaching prosthesis infection environment: Development of an innovative biofilm model.
Fiche publication
Date publication
décembre 2023
Journal
Biofilm
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie, Dr VELARD Frédéric
Tous les auteurs :
Lamret F, Lemaire A, Lagoutte M, Varin-Simon J, Abraham L, Colin M, Braux J, Velard F, Gangloff SC, Reffuveille F
Lien Pubmed
Résumé
The major role and implication of bacterial biofilms in the case of bone and prosthesis infections have been highlighted and often linked to implant colonization. Management strategies of these difficult-to-treat infections consist in surgeries and antibiotic treatment, but the rate of relapse remains high, especially if , a high-virulent pathogen, is involved. Therapeutic approaches are not adapted to the specific features of biofilm in bone context whereas infectious environment is known to importantly influence biofilm structure. In the present study, we aim to characterize SH1000 (methicillin-sensitive strain, MSSA) and USA300 (methicillin-resistant strain, MRSA) biofilm on different surfaces mimicking the periprosthetic environment. As expected, protein adsorption on titanium enhanced the number of adherent bacteria for both strains. On bone explant, USA300 adhered more than SH1000. The simultaneous presence of two different surfaces was also found to change the bacterial behaviour. Thus, proteins adsorption on titanium and bone samples (from bank or directly recovered after an arthroplasty) were found to be key parameters that influence biofilm formation: adhesion, matrix production and biofilm-related gene regulation. These results highlighted the need for new biofilm models, more relevant with the infectious environment by using adapted culture medium and presence of surfaces that are representative of conditions to better evaluate therapeutic strategies against biofilm.
Mots clés
Biofilm, Bone explant, Periprosthetic environment, S. aureus, Titanium
Référence
Biofilm. 2023 12;5:100120