CRISMERE Chromosome Engineering in Mouse and Rat.
Fiche publication
Date publication
janvier 2023
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HERAULT Yann
Tous les auteurs :
Schaeffer L, Lindner L, Pavlovic G, Hérault Y, Birling MC
Lien Pubmed
Résumé
CRISPR/Cas9 technology is a versatile tool for engineering biology that has dramatically transformed our ability to manipulate genomes. In this protocol, we use its capacity to generate two double-strand breaks simultaneously, at precise positions in the genome, to generate mouse or rat lines with deletion, inversion, and duplication of a specific genomic segment. The technic is called CRISMERE for CRISpr-MEdiated REarrangement. This protocol describes the different steps to generate and validate the different chromosomal rearrangements that can be obtained with the technology. These new genetic configurations can be useful to model rare diseases with copy number variation, understand the genomic organization, or provide genetic tools (like balancer chromosome) to keep lethal mutations.
Mots clés
CRISPR/Cas9, Deletion, Droplet digital PCR, Duplication, Inversion, PCR, Sanger sequencing, Structural variants
Référence
Methods Mol Biol. 2023 ;2631:277-297