BF-Azadipyrromethene Fluorophores for Intraoperative Vital Structure Identification.
Fiche publication
Date publication
février 2023
Journal
Molecules (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MARESCAUX Jacques
Tous les auteurs :
Caulfield C, Wu D, Miller IS, Byrne AT, Mac Aonghusa P, Zhuk S, Cinelli L, Bannone E, Marescaux J, Gioux S, Diana M, March TL, Vahrmeijer AL, Cahill R, O'Shea DF
Lien Pubmed
Résumé
A series of mono- and bis-polyethylene glycol (PEG)-substituted BF-azadipyrromethene fluorophores have been synthesized with emissions in the near-infrared region (700-800 nm) for the purpose of fluorescence guided intraoperative imaging; chiefly ureter imaging. The Bis-PEGylation of fluorophores resulted in higher aqueous fluorescence quantum yields, with PEG chain lengths of 2.9 to 4.6 kDa being optimal. Fluorescence ureter identification was possible in a rodent model with the preference for renal excretion notable through comparative fluorescence intensities from the ureters, kidneys and liver. Ureteral identification was also successfully performed in a larger animal porcine model under abdominal surgical conditions. Three tested doses of 0.5, 0.25 and 0.1 mg/kg all successfully identified fluorescent ureters within 20 min of administration which was sustained up to 120 min. 3-D emission heat map imaging allowed the spatial and temporal changes in intensity due to the distinctive peristaltic waves of urine being transferred from the kidneys to the bladder to be identified. As the emission of these fluorophores could be spectrally distinguished from the clinically-used perfusion dye indocyanine green, it is envisaged that their combined use could be a step towards intraoperative colour coding of different tissues.
Mots clés
BF2-azadipyrromethene, NIR-fluorescence, fluorescence guided surgery, pegylation, ureter identification
Référence
Molecules. 2023 02 25;28(5):