Resolution of MoS Nanosheets-induced Pulmonary Inflammation Driven by Nanoscale Intracellular Transformation and Extracellular-vesicle Shuttles.
Fiche publication
Date publication
janvier 2023
Journal
Advanced materials (Deerfield Beach, Fla.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BIANCO Alberto
Tous les auteurs :
Peña NO, Cherukula K, Even B, Ji DK, Razafindrakoto S, Peng S, Silva AKA, Moyon CM, Hillaireau H, Bianco A, Fattal E, Alloyeau D, Gazeau F
Lien Pubmed
Résumé
Pulmonary exposure to some engineered nanomaterials can cause chronic lesions as a result of unresolved inflammation. Among two-dimensional (2D) nanomaterials and graphene, MoS have received tremendous attention in optoelectronics and nanomedicine. Here we propose an integrated approach to follow up the transformation of MoS nanosheets at the nanoscale and their impact on the lung inflammation status over one month after a single inhalation in mice. Analysis of immune cells, alveolar macrophages, extracellular vesicles, and cytokine profiling in bronchoalveolar lavage fluid (BALF) showed that MoS nanosheets induced initiation of lung inflammation that was rapidly resolved despite the persistence of various biotransformed molybdenum-containing nanostructures in alveolar macrophages and extracellular vesicles up to one month. Using in situ liquid phase transmission electron microscopy experiments, we could evidence the dynamics of MoS nanosheets transformation triggered by reactive oxygen species. Three main transformation mechanisms were observed directly at the nanoscale level: 1) scrolling of the dispersed sheets leading to the formation of nanoscrolls and folded patches, 2) etching releasing soluble MoO , and 3) oxidation generating oxidized sheet fragments. Extracellular vesicles released in BALF were also identified as a potential shuttle of MoS nanostructures and their degradation products and more importantly as mediators of inflammation resolution. This article is protected by copyright. All rights reserved.
Mots clés
2D materials, biodegradability, graphene, toxicity, transition metal dichalcogenides
Référence
Adv Mater. 2023 01 17;:e2209615