Intracellular accumulation and immunological response of NIR-II polymeric nanoparticles.
Fiche publication
Date publication
novembre 2022
Journal
International journal of pharmaceutics
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CHARBONNIERE Loïc, Dr KLYMCHENKO Andrey
Tous les auteurs :
Moskalevska I, Faure V, Haye L, Mercey-Ressejac M, Dey AK, Chovelon B, Soro LK, Charbonnière LJ, Reisch A, Klymchenko AS, Marche PN, Coll JL, Macek Jilkova Z, le Guével X
Lien Pubmed
Résumé
Polymeric nanoparticles (NPs) are extremely promising for theranostic applications. However, their interest depends largely on their interactions with immune system, including the capacity to activate inflammation after their capture by macrophages. In the present study, we generated monodisperse poly(ethyl methacrylate) (PEMA) NPs loaded with hydrophobic photoluminescent gold nanoclusters (Au NCs) emitting in the NIR-II optical windows and studied their interaction in vitro with J774.1A macrophages. PEMA NPs showed an efficient time and dose dependent cellular uptake with up to 70 % of macrophages labelled in 24 h without detectable cell death. Interestingly, PEMA and Au-PEMA NPs induced an anti-inflammatory response and a strong down-regulation of nitric oxide level on lipopolysacharides (LPS) activated macrophages, but without influence on the levels of reactive oxygen species (ROS). These polymeric NPs may thus present a potential interest for the treatment of inflammatory diseases.
Mots clés
Gold nanoclusters, Immune response, NIR-II, Nitric oxide
Référence
Int J Pharm. 2022 11 26;630:122439