Absorption Spectra Description for T-Cell Concentrations Determination and Simultaneous Measurements of Species during Co-Cultures.
Fiche publication
Date publication
novembre 2022
Journal
Sensors (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr WACOGNE Bruno
Tous les auteurs :
Wacogne B, Vaccari N, Koubevi C, Belinger-Podevin M, Robert-Nicoud M, Rouleau A, Frelet-Barrand A
Lien Pubmed
Résumé
Advanced Therapy Medicinal Products are promising drugs for patients in therapeutic impasses. Their complex fabrication process implies regular quality controls to monitor cell concentration. Among the different methods available, optical techniques offer several advantages. Our study aims to measure cell concentration in real time in a potential closed-loop environment using white light spectroscopy and to test the possibility of simultaneously measuring concentrations of several species. By analyzing the shapes of the absorption spectra, this system allowed the quantification of T-cells with an accuracy of about 3% during 30 h of cultivation monitoring and 26 h of doubling time, coherent with what is expected for normal cell culture. Moreover, our system permitted concentration measurements for two species in reconstructed co-cultures of T-cells and yeasts. This method can now be applied to any single or co-culture, it allows real-time monitoring, and can be easily integrated into a closed system.
Mots clés
T-cell culture, advanced therapy medicinal product, co-culture monitoring, white light spectroscopy
Référence
Sensors (Basel). 2022 11 27;22(23):