Microsensors and image processing for single oocyte qualification: toward multiparametric determination of the best time for fertilization.
Fiche publication
Date publication
octobre 2013
Auteurs
Membres identifiés du Cancéropôle Est :
Dr AMIOT Clotilde, Dr PAZART Lionel, Dr PIERALLI Christian, Pr ROUX Christophe, Dr WACOGNE Bruno
Tous les auteurs :
Wacogne B, Ivascu I, Zeggari R, Pieralli C, Amiot C, Pazart L, Roux C
Lien Pubmed
Résumé
During intracytoplasmic sperm injection (ICSI) attempts, oocytes reaching metaphase II are microinjected. A morphological examination under a microscope is the usual method for determining oocyte maturity. The level of oocyte maturity is based on the meiotic status (Germinal Vesicle, metaphase I and metaphase II) of the oocytes with respect to their increasing maturity. In this letter, we summarize the studies conducted to analyze cytoplasm maturity using various microsystems and image processing. Optical microsystems are used to measure the transmission spectra and refractive index of the oocytes. We compared the transmission spectra measurements to the transmission electron microscopy results. Karhunen-Loeve transform is also used to evaluate the maturity of the oocytes. To summarize, optical analysis techniques are a minimally invasive technology allowing cytoplasm maturity to be assessed. Oocytes should not only be qualified in terms of GV, MI or MII, but also regarding their temporal evolution over the course of these maturation stages. The ultimate aim of this work is to describe the maturation of the oocytes by a trajectory in a multidimensional space and to determine when would be the best time for successful fertilization.
Référence
Laser Phys Lett. 2013 Oct;10(10):105601.