Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses.
Fiche publication
Date publication
décembre 2022
Journal
Journal of ovarian research
Auteurs
Membres identifiés du Cancéropôle Est :
Pr FAUQUE Patricia
Tous les auteurs :
Barberet J, Ducreux B, Bruno C, Guilleman M, Simonot R, Lieury N, Guilloteau A, Bourc'his D, Fauque P
Lien Pubmed
Résumé
Indications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. In this study, we evaluated the efficiency of the semi-automated technique of oocyte vitrification by survival rate, morphometric assessment and resistance to empty micro-injection gesture as compared with a manual method. Additionally, we intended to evaluate transcriptomic consequences of both techniques using single-cell RNA-seq technology.
Mots clés
Assisted reproductive technology, Cryopreservation, Oocyte, Single-cell RNA-seq, Vitrification
Référence
J Ovarian Res. 2022 12 5;15(1):128