3D differential interference contrast microscopy using polarisation sensitive tomographic diffraction microscopy.
Fiche publication
Date publication
novembre 2022
Journal
Journal of microscopy
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HAEBERLE Olivier
Tous les auteurs :
Verrier N, Taddese AM, Abbessi R, Debailleul M, Haeberlé O
Lien Pubmed
Résumé
Tomographic Diffraction Microscopy (TDM) is a generalisation of Digital Holographic Microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3-D, high-resolution imaging of unlabelled samples. TDM makes it possible to obtain the optical field in both amplitude and phase for each illumination angle. Proper information reallocation eventually allows for 3D reconstruction of the complex refractive index map. On the other hand, Polarisation Array Sensors (PAS) paves new way for TDM, as vectorial information assessment about the investigated sample. In this contribution, we show an alternative use of this polarisation information based on the phase sensitive nature of TDM. Here, we demonstrated that TDM coupled with PAS can lead to a 3D Differential Interference Contrast (DIC) microscope with almost no experimental configuration modification. This article is protected by copyright. All rights reserved.
Mots clés
holography, microscopy, tomography
Référence
J Microsc. 2022 11 21;: