Dual-Color Photoconvertible Fluorescent Probes Based on Directed Photooxidation Induced Conversion for bioimaging.
Fiche publication
Date publication
novembre 2022
Journal
Angewandte Chemie (International ed. in English)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr KLYMCHENKO Andrey
Tous les auteurs :
Saladin L, Breton V, Dal Pra O, Klymchenko AS, Danglot L, Didier P, Collot M
Lien Pubmed
Résumé
We herein present a new concept to produce dual-color photoconvertible probes based on a mechanism called Directed Photooxidation Induced Conversion (DPIC). As a support of this mechanism, styryl-coumarins (SCs) bearing Aromatic Singlet Oxygen Reactive Moieties (ASORM) like furan and pyrrole have been synthesized. SCs are bright fluorophores, which undergo a hypsochromic conversion upon visible light irradiation due to directed photooxidation of the ASORM that leads to the disruption of conjugation. SC-P, a yellow emitting probe bearing a pyrrole moiety, converts to a stable blue emitting coumarin with a 68 nm shift allowing the photoconversion and tracking of lipid droplet in live cells. This new approach might pave the way to a new generation of photoconvertible dyes for advanced bioimaging applications.
Mots clés
Fluorescent probes, Photoconversion, Photooxidation, Photoswitching, lipid droplets
Référence
Angew Chem Int Ed Engl. 2022 11 24;: