Long term culture of mesenchymal stem cells in hypoxia promotes a genetic program maintaining their undifferentiated and multipotent status.
Fiche publication
Date publication
mars 2011
Journal
BMC cell biology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DE CARVALHO BITTENCOURT Marcelo, Dr DE ISLA Natalia
Tous les auteurs :
Basciano L, Nemos C, Foliguet B, de Isla N, de Carvalho M, Tran N, Dalloul A
Lien Pubmed
Résumé
In the bone marrow, hematopietic and mesenchymal stem cells form a unique niche in which the oxygen tension is low. Hypoxia may have a role in maintaining stem cell fate, self renewal and multipotency. However, whereas most studies addressed the effect of transient in vitro exposure of MSC to hypoxia, permanent culture under hypoxia should reflect the better physiological conditions.
Mots clés
Cell Count, Cell Differentiation, physiology, Cell Hypoxia, Cell Proliferation, Cells, Cultured, Flow Cytometry, Gene Expression, Gene Expression Profiling, Gene Expression Regulation, Humans, Mesenchymal Stem Cells, cytology, Microscopy, Electron, Multipotent Stem Cells, cytology, Stem Cell Research
Référence
BMC Cell Biol.. 2011 Mar 30;12:12