SHG as a new modality for large field of view imaging to monitor tissue collagen network.
Fiche publication
Date publication
janvier 2012
Journal
Bio-medical materials and engineering
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DUMAS Dominique, Pr MAGDALOU Jacques, Pr MENU Patrick, Dr DE ISLA Natalia
Tous les auteurs :
Dumas D, Hupont S, Huselstein C, de Isla N, Rousseau M, Werkmeister E, Magdalou J, Menu P, Stoltz JF
Lien Pubmed
Résumé
For this study, we have considered a new large field of view imaging dedicated to matrix collagen (no stained samples). To integrate a multidimensional scale (non-sliced samples), a femtosecond oscillator (two photon excitation laser) has been coupled with a large field optical setup to collect SHG signal. We introduced an index (F-SHG) based on decay time response measured by TCSPC for, respectively, Fluorescence (F) and Second Harmonic Generation (SHG) values. For samples where protein collagen is the major component of extracellular matrix (skin) or not (nacre), we compared the index distribution (from 2 to 12) obtained with large field optical setup. In this work, we showed for the first time that multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) could be an innovative and non invasive technique to detect and identify some biological interest molecules (collagen) in biomedical topics.
Mots clés
Animals, Collagen, analysis, Extracellular Matrix, chemistry, Male, Microscopy, Fluorescence, Multiphoton, methods, Nacre, analysis, Pinctada, chemistry, Rats, Skin, chemistry
Référence
Biomed Mater Eng. 2012 ;22(1-3):159-62